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基本信息

质粒图谱和多克隆位点信息

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载体描述

在pCAMBIA1300-35S的基础上，加入EGFP蛋白序列，便于定位和检测。

载体序列

ORIGIN

        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt

       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa

      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc

      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt

      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt

      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc

      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc

      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc

      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc

      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc

      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac

      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca

      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca

      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag

      841 agaacacggg ggacgagctc ggtacccggg gatcctctag agtcgacatg gtgagcaagg

      901 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg

      961 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc

     1021 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc

     1081 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct

     1141 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg

     1201 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg

     1261 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca

     1321 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga

     1381 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc

     1441 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc

     1501 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg

     1561 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaactg cagagctttc

     1621 gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac

     1681 acaccagaat cctactgagt tcgagtatta tggcattggg aaacatgttt ttcttgtacc

     1741 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa

     1801 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa

     1861 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaaatataa gagatatgca

     1921 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat

     1981 gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt

     2041 tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac

     2101 atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt

     2161 tataattata gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt

     2221 ttatgacttg ccaattgatt gacaacatgc atcaagcttg gcactggccg tcgttttaca

     2281 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc

     2341 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg

     2401 cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct

     2461 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag

     2521 cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt

     2581 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc

     2641 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg

     2701 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt

     2761 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc

     2821 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag

     2881 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag

     2941 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc

     3001 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta

     3061 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg

     3121 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc

     3181 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc

     3241 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc

     3301 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat

     3361 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg

     3421 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag

     3481 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt

     3541 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg

     3601 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg

     3661 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat

     3721 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa

     3781 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg

     3841 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga

     3901 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga

     3961 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc

     4021 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt

     4081 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat

     4141 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag

     4201 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt

     4261 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt

     4321 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga

     4381 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa

     4441 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca

     4501 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc

     4561 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc

     4621 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa

     4681 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca

     4741 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt

     4801 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc

     4861 ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc

     4921 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac

     4981 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc

     5041 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt

     5101 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg

     5161 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag

     5221 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc

     5281 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg

     5341 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc

     5401 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca

     5461 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg

     5521 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt

     5581 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg

     5641 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct

     5701 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct

     5761 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga

     5821 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga

     5881 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta

     5941 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc

     6001 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca

     6061 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag

     6121 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc

     6181 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta

     6241 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg

     6301 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc

     6361 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat

     6421 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg

     6481 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc

     6541 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat

     6601 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa

     6661 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg

     6721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag

     6781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc

     6841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca

     6901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt

     6961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc

     7021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc

     7081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc

     7141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact

     7201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg

     7261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta

     7321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca

     7381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa

     7441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg

     7501 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta

     7561 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct

     7621 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat

     7681 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt

     7741 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct

     7801 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc

     7861 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc

     7921 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga

     7981 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg

     8041 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa

     8101 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc

     8161 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt

     8221 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc

     8281 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt

     8341 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac

     8401 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc

     8461 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg

     8521 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat

     8581 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg

     8641 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg

     8701 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt

     8761 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg

     8821 acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg

     8881 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca

     8941 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc

     9001 ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc

     9061 cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact

     9121 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg

     9181 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca

     9241 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg

     9301 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta

     9361 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc

     9421 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt

     9481 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca

     9541 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc

     9601 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg

     9661 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct

     9721 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag

     9781 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt

     9841 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc

     9901 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag

     9961 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag

    10021 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc

    10081 ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag ctcgagagag

    10141 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc

    10201 cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt

    10261 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac

    10321 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac

    10381 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt

    10441 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac

    10501 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt

    10561 ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt gctttgaaga

    10621 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg

    10681 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt

    10741 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg

    10801 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc

    10861 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt

    10921 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa

    10981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat

    11041 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg

    11101 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattac

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