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pDC316-mCMV-EGFP 构建费用500元/个起

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基本信息

体类型:

腺病毒表达载体Admax系列表达质粒

载体抗性:

氨苄青霉素 

载体标签

AD5

筛选标记:

/

启动子

mCMV promoter

表达水平:

高拷贝

载体大小:

5 879bp

克隆方法:

多克隆位点,限制性内切酶

5' 测序引物:

T7 primer: 5-TAATACGACTCACTATAGGG-3

3' 测序引物

BGH-W: 5-GGGAGGGGCAAACAACAGATG-3

组成型:

组成型

备注

腺病毒Admax系列表达质粒

质粒图谱和多克隆位点信息


注意:

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如要使用本公司保存的母源载体时,本公司不能保证所有母源载体完全符合客户预期,也不承担由此产生的任何责任。

载体描述 

pDC316-mCMV-EGFP是一个腺病毒Admax系列表达质粒。

 

载体序列

LOCUS       Exported                5879 bp ds-DNA     circular SYN 29-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pDC316-mCMV-EGFP

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5879)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Friday, Mar 29, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..5879

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    2..102

                     /label=terminal repeat

                     /note="inverted terminal repeat"

     misc_signal     190..340

                     /label=Ad5 Psi

                     /note="packaging signal for adenovirus serotype 5"

     misc_feature    468..982

                     /label=mCMV promoter

                     /note="mcmv promoter (-491 to +36)"

     CDS             999..1715

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     polyA_signal    1862..1983

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     enhancer        1991..2370

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        2371..2574

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early

                     promoter"

     primer_bind     2619..2638

                     /label=T7 primer

     promoter        2619..2637

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     misc_feature    2651..2740

                     /label=MCS

                     /note="multiple cloning site"

     polyA_signal    2764..2988

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     primer_bind     complement(2785..2805)

                     /label=BGH-W

     polyA_signal    3002..3136

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     protein_bind    3156..3189

                     /allele="Cre recombinase"

                     /allele="Cre-mediated recombination occurs in the 8-bp core

                      sequence (GCATACAT)"

                     /label=loxP

                     /bound_moiety="Cre recombinase"

                     /note="Cre-mediated recombination occurs in the 8-bp core

                     sequence (GCATACAT)."

     primer_bind     complement(3211..3227)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    3235..3251

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(3259..3289)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    3304..3325

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(3613..4201)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(4372..5232)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5233..5337)

                     /gene="bla"

                     /label=AmpR promoter

     misc_feature    join(5420..5879,1..453)

                     /label=AD

                     /note="recombinant human adenovirus type 5 (E1/E3 deleted)

                     expressing enhanced green fluorescent protein"

     misc_feature    join(5420..5879,1..2)

                     /label=terminator

                     /note="terminal repeat"

     misc_feature    join(5789..5879,1)

                     /label=terminal repeat

                     /note="inverted terminator repeat"

ORIGIN

        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt

       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg

      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg

      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt

      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa

      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg

      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc

      421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga

      481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc

      541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa

      601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat

      661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc

      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat

      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg

      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct

      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca

      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcat ggtgagcaag ggcgaggagc

     1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt

     1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca

     1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg

     1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg

     1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca

     1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg

     1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca

     1441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga

     1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc

     1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc

     1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg

     1681 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagatccac

     1741 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt

     1801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt

     1861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac

     1921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc

     1981 ttaacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta

     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc

     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg

     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg

     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa

     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac

     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg

     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg

     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca

     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg

     2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag

     2641 acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc actgtgctgg

     2701 atatctgcag aattgatccg agctcggtac caagcttaag tttaaaccgc tgatcagcct

     2761 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga

     2821 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt

     2881 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg

     2941 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt cgacttcgag

     3001 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac

     3061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc

     3121 ttatcatgtc tggatcgtct agcatcgaag atccaataac ttcgtatagc atacattata

     3181 cgaagttata agtagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta

     3241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc

     3301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg

     3361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg

     3421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg

     3481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa

     3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc

     3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc

     3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag

     3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct

     3781 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta

     3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc

     3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc

     3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt

     4021 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct

     4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc

     4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca

     4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta

     4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa

     4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg

     4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg

     4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc

     4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc

     4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa

     4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc

     4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg

     4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc

     4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat

     4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg

     4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc

     4981 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg

     5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat

     5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg

     5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg

     5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct

     5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac

     5341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta

     5401 taaaaatagg cgtatcactc taggcaaaat agcaccctcc cgctccagaa caacatacag

     5461 cgcttcacag cggcagccta acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa

     5521 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag

     5581 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag

     5641 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa

     5701 tcgtcacttc cgttttccca cgttacgtaa cttcccattt taagaaaact acaattccca

     5761 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg

     5821 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtata

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