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基本信息

质粒图谱和多克隆位点信息 

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载体描述 

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.  These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters.  Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone.

     The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies. A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies.

      • Express two different genes simultaneously in S. cerevisiae 
      • Proteins tagged with unique epitope tags 
      • Fast and easy immunoprecipitations

载体序列

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc

      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca

      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatataaa

      361 ttaggaatca tagtttcatg attttctgtt acacctaact ttttgtgtgg tgccctcctc

      421 cttgtcaata ttaatgttaa agtgcaattc tttttcctta tcacgttgag ccattagtat

      481 caatttgctt acctgtattc ctttacatcc tcctttttct ccttcttgat aaatgtatgt

      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg

      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct

      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg

      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct

      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac

      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat

      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc

      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg

     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca

     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc

     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata

     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact

     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc

     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca

     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt

     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca

     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg

     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca

     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga

     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc

     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata

     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat

     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat

     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct

     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca

     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat

     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga

     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg

     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt

     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg

     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta

     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa

     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt

     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa

     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag

     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt

     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac

     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag

     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg

     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg

     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc

     3001 cagctgaatt ggagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga

     3061 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac

     3121 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta

     3181 tttagaagtg tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat

     3241 ttctggcaag gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa

     3301 gaattgttaa ttaagagctc agatcttatc gtcgtcatcc ttgtaatcca tcgatactag

     3361 tgcggccgcc ctttagtgag ggttgaattc gaattttcaa aaattcttac tttttttttg

     3421 gatggacgca aagaagttta ataatcatat tacatggcat taccaccata tacatatcca

     3481 tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc

     3541 ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta

     3601 tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc

     3661 tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca

     3721 ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt

     3781 ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg

     3841 ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt

     3901 ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc

     3961 aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg

     4021 ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatccgt aatacgactc

     4081 actatagggc ccgggcgtcg acatggaaca gaagttgatt tccgaagaag acctcgagta

     4141 agcttggtac cgcggctagc taagatccgc tctaaccgaa aaggaaggag ttagacaacc

     4201 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat

     4261 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa

     4321 aaccttgctt gagaaggttt tgggacgctc gaagatccag ctgcattaat gaatcggcca

     4381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc

     4441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg

     4501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa

     4561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga

     4621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag

     4681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct

     4741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg

     4801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc

     4861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt

     4921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta

     4981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac

     5041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc

     5101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat

     5161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc

     5221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt

     5281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta

     5341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct

     5401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg

     5461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga

     5521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt

     5581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt

     5641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt

     5701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat

     5761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc

     5821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc

     5881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat

     5941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag

     6001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt

     6061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc

     6121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa

     6181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg

     6241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa

     6301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg

     6361 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc

     6421 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa

     6481 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa

     6541 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca

     6601 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa

     6661 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata

     6721 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc

     6781 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg

     6841 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat

     6901 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg

     6961 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt

     7021 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga

     7081 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga

     7141 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa

     7201 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg

     7261 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta

     7321 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg

     7381 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat

     7441 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt

     7501 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg

     7561 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt

     7621 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg

     7681 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta

     7741 tcacgaggcc ctttcgtc

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